We identify a mechanism for biological spatial pattern formation arising when the signals that mediate interactions between individuals in a population have pulsed character. Our general population-signal framework shows that while for a slow signal-dynamics limit no pattern formation is observed for any values of the model parameters, for a fast limit, on the contrary, pattern formation can occur. Furthermore, at these limits, our framework reduces, respectively, to reaction-diffusion and spatially nonlocal models, thus bridging these approaches.