presentation/233 1st BioSim Conference - Presentation

1st BioSim Conference


Study of drug metabolism of chloramphenicol and propiverine in yeast cells under aerobic and anaerobic conditions

Author: Jörg Smolinski, Manfred Bräter and Martin Bertau, Technical University Dresden, Institute of Biochemistry.

Names and affiliation of other authors:
Jörg Smolinski(1), Manfred Bräter(2) and Martin Bertau(1)
1 Technische Universität Dresden, Institut für Biochemie, 01062 Dresden, Germany
2 APOGEPHA Arzneimittel GmbH, Kyffhäuserstraße 27, 01309 Dresden, Germany

Oral or poster: Poster

Saccharomyces cerevisiae serves as model organism in the flux cell model described by Brusch et al. to predict the metabolic fate of pharmaceutical active compounds in mammalian cells. Therefore the investigation of the different metabolic profiles corresponding to different bioransformation conditons is essentiell.
Chloramphenicol and propiverine hydrochloride were submitted to biotransformation by Saccharomyces cerevisiae with purpose of investigating potential effects of aerobicity and anaerobicity, respectively, on cell metabolism as well as its outcome on biotransformation product distribution.
Additionally the influence of sugar limitation on the metabolic profile was analysed.
The sum of these data provide new insight into drug metabolism by living cells.

Brusch L. et. al., Biophys Chem. 109(3) 2004, 413-426

Formatted version of the abstract or additional information

1st BioSim Conference. October 6th to October 8th, 2005. Palma de Mallorca, Spain.