presentation/216 1st BioSim Conference - Presentation

1st BioSim Conference

Presentation

Comparison of cell based and membrane based high throughput assays for the detection of drugs interacting with the BCRP transporter

Author: Szilvia Gedey, SOLVO Biotechnology.

Names and affiliation of other authors:
Hristos Glavinas, Emese Kis, Ákos Pál, Dóra Méhn, Márton Jani, Berend Oosterhuis, Tünde Nagy, György Báthori, Péter Krajcsi
SOLVO Biotechnology, Szeged, Hungary

Oral or poster: Poster

Abstract:
The aim of this study was to compare different in vitro assays used to screen for BCRP interacting compounds.Cellular assays only detected low permeability substrates, while they failed to detect high permeability compounds.Our novel ATPase assay detected all substrates as activators of the BCRP ATPase activity. The direct vesicular transport assay only detected the transport of low permeability compounds, while the indirect vesicular transport assay detected all known substrates and inhibitors.

1st BioSim Conference. October 6th to October 8th, 2005. Palma de Mallorca, Spain.